产品说明(Description)
Recombinant Human Interleukin-15 is produced by our E.coli expression system and the target gene encoding Asn49-Ser162 is expressed.
Accession #: P40933
Known as: Interleukin-15; IL-15; IL15
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL test.
Bioactivity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.
The ED50 for this effect is 40-200pg/ml.
The ED50 for this effect is typically 99.94 pg/mL.（Novoprotein）
The ED50 for this effect is typically 346.61pg/mL. (R)
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Human Interleukin 15 (IL-15) is a cytokine that regulates T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL15 receptor (IL-15RA) with high affinity. IL-15 also binds to the beta and gamma chains of the IL-2 receptor, but not the alpha subunit of the IL2 receptor. IL-15 is structurally and functionally related to IL-2. Both cytokines share some subunits of receptors, allowing them to compete for and negatively regulate each other's activity. The number of CD8+ memory T cells is controlled by a balance between IL-15 and IL-2. Despite their many overlapping functional properties, IL-2 and IL-15 are, in fact, quite distinct players in the immune system. IL-15 is constitutively expressed by a wide variety of cell types and tissues, including monocytes, macrophages and DCs. Mature Human IL-15 shares 70% amino acid sequence identity with Mouse and Rat IL-15.
电泳(SDS-PAGE)
Cas9-Cleavage Sequences in Size-Reduced Plasmids Enhance Nonviral Genome Targeting of CARs in Primary Human T Cells[J]. Ruirui Jing. et al. Small methods. 2021.
MUC1-Tn-targeting chimeric antigen receptor-modified Vγ9Vδ2 T cells with enhanced antigen-specific anti-tumor activity[J]. Xiaochen Zhai. et al. American Journal of Cancer Research. 2021.
Next Generation Sequencing- Based Identification of T- Cell Receptors for Immunotherapy Against Hepatocellular Carcinoma[J]. Yipeng Ma. et al. Clinical and Translational Medicine. 2021.
Identification of neoantigen-specific T cells and their targets: implications for immunotherapy of head and neck squamous cell carcinoma[J]. Lili Ren. et al. OncoImmunology. 2019.
Induction of Neoantigen-specific Cytotoxic T Cells and Construction of T-cell Receptor-engineered T cells for Ovarian Cancer[J]. Tatsuo Matsuda. et al. Clinical Cancer Research. 2018.
Discovery of a novel IL-15 based protein with improved developability and efficacy for cancer immunotherapy[J]. Qiyue Hu. et al. Scientific Reports. 2018.
Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells[J]. Taigo Kato. et al. Oncotarget. 2018.
Novel anti-GD2 CAR-T cells exhibit superior cytotoxicity against neuroblastoma[J]. Cheng ji. et al. European Journal of Inflammation. 2020.
